產品基本信息
產品名稱：VIC 染料 qPCR 校準溶液 *10,000X*
儲存條件：-15℃避光防潮
保質期：12個月
產品物理化學光譜特性
分子量：NA
溶劑：水
激發波長(nm)：526
發射波長(nm)：543
產品介紹
實時熱循環儀需要校準才能生成熒光信號的光譜圖。由于儀器的熒光檢測靈敏度可能不同，校準任何染料（如 VIC）所需的濃度將取決于所使用的設備。 AAT Bioquest VIC 染料 qPCR 校準溶液以 10,000X 濃縮液形式提供，可與多種 qPCR 儀器兼容，包括 ABI7500 Fast、QuantStudio? 和 ViiA? 7 系統。校準和驗證應每六個月或根據需要運行一次。百螢生物是AAT Bioquest的中國代理商，為您提供優質的VIC 染料 qPCR 校準溶液 *10,000X*。
實驗方案
此通用協議適用于 ABI7500 FAST qPCR 儀器。
準備 VIC 校準板：
1.將 1 μL 的 10,000X VIC 染料 qPCR 校準溶液與 10 mL 的無核酸酶水或 qPCR 緩沖液混合，制備 VIC 染料 qPCR 校準工作溶液。請注意，染料濃度必須根據儀器的校準要求進行優化。
2.在反應板的所有孔中加入 20 μL 的 VIC Dye qPCR 校準工作溶液。
3.用光學膠蓋密封板
4.以 < 1500 rpm 的速度將板離心 2 分鐘
5.檢查板的每個孔中的液體是否位于孔的底部。如果沒有，請再次以稍高的 rpm 和更長的時間將板離心
6.有關完整的校準說明，請參考您儀器的維護指南
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